Aging/Search log

a.AGINGRESEARCH IN PROGRESS

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An evolving AI research projectBackground · goals · methodsUpdated 15 Oct 2026

Search log

Connect the literature. Challenge assumptions. Understand function.

Cumulative update · 15 Oct 2026 ARTISTA documents an analysis resource for Wei 2022. Filenames containing “rep1–4” do not establish independent animals. The critical mapping is still unverified; no new functional confirmation follows. AG-C06 and AG-T01 remain 🔵 Level 2. Daily report 15 Oct 2026 →

Search · 15 Oct 2026

Targeted follow-up to the unresolved AG-S02 metadata check: six bounded read-only GitHub calls, including one unsupported README endpoint; the specific file reader subsequently succeeded. README and tree examined; no clone, code execution, matrices or CSV contents. No new broad literature or notice search today; the bounded DOI correction/retraction search of 14 October is not presented as today’s check. New-study delta 0; still 29 studies. Unverified metadata do not establish that such data or functional assays are absent from the complete study.

Search · 14 Oct 2026

Targeted AG-S02 assessment of DOI, original main text, figure legends and injury/timing details; notice check and precise access limits in today’s report. Current search “TIMP2 axolotl brain regeneration aging October 2026 study” with a 7-day filter returned known papers, reviews, secondary reporting and unappraised leads. Direct iSpheroid PubMed entry 42856336 provided no usable article text; DOI/version unverified. Regional brain aging 42843341 and Bonn reprogramming news screened only. No scientific results admitted from these unappraised leads; no complete coverage. Source delta 0. AG-S02 DOI correction/retraction search found no attributable notice, non-exhaustive. Publisher access403, PubMed access limited; identified publicly hosted PDF copy, host authorization unverified. Supplement/pixels not audited.

Search · 13 Oct 2026

Current search “axolotl regeneration brain aging TIMP2 October 2026 original study” with a 14-day filter returned known and unappraised adjacent results; no comprehensive coverage. Amamoto 2016 selected as a relevant historical counterexample. Direct publisher request returned a JS challenge, PMC a reCAPTCHA and Harvard PDF a retrieval error; no bypass. Targeted official indexed original passages assessed for Figure 7/connectivity and electrophysiological results; Harvard metadata confirms provenance. DOI correction/retraction search found no attributable notice, non-exhaustive. AG-D06 institutional PDF read once again: same previously known locator, no new empirical finding; previous provenance wording corrected. Registry delta +1 (AG-D07).

Search · 12 Oct 2026

New targeted check of GSM9001954/55 and WT bulk comparator GSM9001961: official GEO index provides 1954 in full, only series labels for the others; field search empty, newly targeted direct sample requests returned cache misses. No repeated old challenges or raw-data retrieval. INTACT-olotl axolotl lead screened: PubMed without usable text; candidate DOI 10.64898/2026.09.18.752769 and preprint label only in a secondary index, bioRxiv original inaccessible and targeted domain search empty. No scientific results admitted from these summaries. Current search “TIMP2 2026 October aging microglia” with a 14-day filter returned known and unappraised adjacent papers plus a new institutional PDF locator for AG-D06. PDF not opened today; next targeted evidence route. AG-R01 correction/retraction check of 10 October not repeated without a trigger. Source delta 0, no complete temporal coverage claimed.

Search · 11 Oct 2026

Targeted audit of GEO GSE297916/17 and GSM9001952/53/60 using indexed official metadata; direct GEO access returned reCAPTCHA and a Nature methods request was limited by an IDP redirect. No identical retries, matrix downloads or complete new methods appraisal. The AG-R01 correction/retraction check of 10 October was not repeated without a trigger; no new claim of completeness. Current search “axolotl regeneration October 2026 original study” with a 14-day filter returned registered papers, a news feature and the unappraised methods lead “INTACT-olotl” (PubMed 42818152; indexed date 22 September 2026). DOI, version and methods of this lead remain unverified; not incorporated as evidence. Source delta 0; no claim of complete coverage.

Search · 10 Oct 2026

New question after the 9 October audit: author/institution lists and dependency statements for AG-R05/08/01. Targeted original-source sections checked; no repeated complete effect appraisal or raw-data analysis. DOI searches for AG-R01/08 corrections/retractions found no attributable new notice; yesterday’s AG-R05 search was not repeated. Current search “TIMP2 aging October 2026 regeneration study” (14-day filter) returned adjacent leads without methods appraisal. Search filters/index dates are not publication dates. No source added; latest selected first publication remains 26 August 2026. No claim of complete coverage.

Search · 9 Oct 2026

Targeted AG-R05 methods audit following the open step identified on 8 October. Direct PMC access was limited by reCAPTCHA and publisher access by a redirect; no identical retries. Two targeted queries yielded indexed original passages. Also searched DOI+correction/retraction and TIMP2+2026+October: no attributable new correction/retraction notice or new study ready for inclusion found. Hits concerning other papers were not attributed to this study. An institutional profile hit remains an unappraised lead without confirmed DOI/publication metadata. Not comprehensive monitoring; latest selected first publication remains 26 August 2026. Source delta 0; no fresh axolotl audit without a new trigger.

Search · 8 Oct 2026

Reason: open AG-D06 APOE/cis/LD/colocalisation check. Direct PMC request and observed supplement reference return reCAPTCHA today; no specific supplement file read. Three targeted DOI/PMCID+supplement/code queries yielded no verified article-specific code link. Indexed AppendixA points only to the DOI. Earlier publisher/PDF403 not retried. Main run: DOI+correction/retraction and TIMP2 cognition2026 September study; no matching notice, mostly unrelated cognition results, no extra study admitted. No completeness guarantee. Source delta0. Access gap parked pending concrete new access; next separate source check AG-R05.

Search · 7 Oct 2026

Reason: human PRS candidate queued6Oct. Searched DOI and DOI+correction/retraction; assessed final PMC main text, institutional PDF text and PubMed metadata. First online23Oct2025, issueJanuary2026, retrieved7Oct2026. No matching correction/retraction found; no completeness guarantee. PubMed links medRxiv10.1101/2024.07.23.24310854 and ResearchSquare10.21203/rs.3.rs-5267673/v1 as predecessors, not independent evidence. ScienceDirect403 not retried; institutional download403 not repeated. Pixel inspection unsuccessful; tables read as text, supplement/raw data unexamined. No new effect calculation. Source delta+1(AG-D06); no repeat broad axolotl search. Next: inspect existing APOE/cis/LD/colocalisation checks in supplement/code.

Search · 6 Oct 2026

Reason: open direct-group comparison in AG-R08. Indexed eNeuro/PMC original methods, Fig.1/6 legends and conflicts examined; figure subpage unavailable directly, old PMC CAPTCHA not retried. No figure pixels/raw data assessed. Searched DOI with correction/retraction and TIMP2 cognition synapse2026 September; no matching correction notice, no completeness guarantee. Publisher/PMC date discrepancy unresolved; extra date search did not reliably clarify it. One human PRS candidate (10.1016/j.neurobiolaging.2025.10.003) screened from indexed text only; direct publisher403, no retry; exact first date, methods/dependencies next. Source delta0. No own effect calculation; p-values are published statistics.

Search · 5 Oct 2026

Reason: open audit from 4 Oct. Official AG-S03 peer-review file downloaded normally (45 pages), full extracted text searched for Nacu/2016, SAG, ALM, sorting/selection, EGFP, functional; relevant sections read and PDF pages 4, 5, 17, 18, 27, 28, 29, 31, 35, 36 visually checked. Not a line-by-line audit of the whole file. No matched direct Nacu-ED3 comparison found; negative search finding limited to this scope. Revision-letter abnormal digits 4/6 lack final figure/case linkage. Targeted title/DOI correction/retraction check found no matching notice in publisher/PubMed/PMC results; no completeness guarantee. Source delta 0, no new study selected today, no broad search for latest publications or repeated inaccessible tau full texts. No calculation without case linkage. Original file

Search · 4 Oct 2026

Specific reason: unresolved Nacu/Otsuki disagreement from 3 Oct. Exact title/DOI 10.1038/nature17972, primary preview, Extended Data 3 and original supplement inspected; main text from a ResearchGate copy labelled as author-uploaded, without authenticated version comparison. ED3-specific exposure and graft/animal dependencies remain open. AG-S03 methods and Extended Data 10 reviewed for washout/endpoint and cell selection. Targeted Nacu DOI correction/retraction search found no matching notice; not a complete registry audit, commentaries are not corrections. One older primary study added; no new publication today or repeated broad tau search. No cross-study calculation.

Search · 2–3 Oct2026

2 Oct: Jiang DOI/original abstract, version and correction/retraction searches; final publisher 403, no access bypass. Guo reporting form visually inspected. 3 Oct: Crossref/bioRxiv for Jiang versions without explicit relation; author comparison with Guo. AG-S03 full text Figs. 3–5, discussion/methods targeted for spatial control; DOI+correction/retraction found no matching notice (not comprehensive registry audit). Nacu 2016 identified for next countercheck, not fully appraised today. No repeated numerical analysis, no new study on3 Oct; retrieval and publication dates kept separate.

Search · 1 Oct 2026

AG-D01: targeted fragment/epitope audit, main text accessible; supplement errors, no bypass. AG-D03: autophagy/antibody/LC3 and primary DOI; publisher preview, figures and public XLSX inspected. Descriptive rotarod recalculation, not independent replication. DOI/title correction/retraction search found no matching notice; not a complete registry audit. Related DOI 10.1126/scitranslmed.aea4205 identified only as the next appraisal candidate, not counted as deeply reviewed today.

Search · 30 Sep 2026

One additional paper (AG-D02), deeper measurement audit of AG-D01. Searched microglia/tau/degradation/export and quantitative assays; DOI/title plus correction/retraction found no matching notice, not a full registry audit. Shultz: indexed primary methods and figure legends, direct retrieval blocked; preprint not counted separately. Karabova: Figure 4 and methods assessed, supplement access limited. No verified matched dataset for calculating degradation rates.

Search · 29 Sep 2026

Today: targeted methods review of two existing papers and one additional primary study, three sources in total. DOI/title correction and retraction searches found no matching notice; no comprehensive registry audit. Guldner: direct retrieval blocked, indexed publisher sections read. Hemmer: PMC methods read, supplementary figure inaccessible. Karabova: publisher full text accessible. Publication dates are separated from retrieval date in the report. No systematic September coverage.

Targeted narrative search of publisher pages, PubMed and PMC; not a systematic review or meta-analysis. Fourteen primary studies: thirteen peer-reviewed and one preprint. First-publication dates in the selected set reach June 2026; later material has not been comprehensively checked. Older mechanistic studies were retained. No original raw-data analysis or experiments were performed.

Search queries and follow-up verification

• axolotl regeneration 2026 2025 brain protein ageing regeneration primary study
• brain aging protein 2026 rejuvenation Nature study
• axolotl senescence limb regeneration 2025 2026 study
• site.nature.com brain 2025 aging protein Nature original study
• Shifts in protein aggregate stability 2026
• site.nature.com KLK6 2026 aging brain
• site.science.org brain aging microglia 2026 midlife 3D genome
• Axolotl brain 2022 Science regeneration single cell
• Ageing promotes microglial Published
• FTL1 aging hippocampus 2025 Villeda Nature Aging
• axolotl heart regeneration 2025 2026 original study
• Molecular basis positional memory limb regeneration Otsuki 2025 09036
• FTL1 deubiquitinase neuronal aging USP7 redox
• FTL1 deubiquitinase ferritin aging brain DUB
• "Ftl1" "deubiquitylase"
• "FTL1" "USP7" aging

Title, DOI, date and source access were checked separately. The targeted FTL1/DUB prior-art search did not establish novelty. The June 2026 cardiac study was followed up via PMID 42346394. Press and social sources were not used as outcome evidence. Some PubMed/PMC/Elsevier pages were challenge-protected; source-level access limitations are in the register. Full supplements, all power analyses, conflicts of interest and raw data were not audited. Independent replication was generally not assessed. Clinical intervention studies and trial registers were outside this first mechanistic report; their omission is not an assessment of the clinical field.

Next run: new studies, corrections and independent replications; a focused test of the FTL1/redox/DUB hypothesis. Preserve negative searches and conflicting evidence.

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a.AGINGRESEARCH IN PROGRESS

Project overviewGoals & methodsEvidence chain

AI-assisted literature research and model building. No laboratory or clinical studies conducted by this project. No personal treatment plans.

Image credits · Illustrations, not project data: Axolotl — LoKiLeCh (CC BY-SA 3.0) · Neuron — explorebiology (Explore Biology) (CC BY 4.0) · DNA — Biochemlife (CC BY-SA 4.0) · Bovine endothelial cells — Erin Rod (CC BY 4.0)