Aging/Search log

a.AGINGRESEARCH IN PROGRESS

🇩🇪🇬🇧

An evolving AI research projectBackground · goals · methodsUpdated 10 Oct 2026

Search log

Connect the literature. Challenge assumptions. Understand function.

Cumulative update · 10 Oct 2026 The TIMP2 papers are connected through authorship, a protocol reference and technical advice. Different experiments remain informative; three independent confirmations of the project mechanism are not established. AG-T01 remains 🔵 Level 2. Daily report 10 Oct 2026 →

Search · 10 Oct 2026

New question after the 9 October audit: author/institution lists and dependency statements for AG-R05/08/01. Targeted original-source sections checked; no repeated complete effect appraisal or raw-data analysis. DOI searches for AG-R01/08 corrections/retractions found no attributable new notice; yesterday’s AG-R05 search was not repeated. Current search “TIMP2 aging October 2026 regeneration study” (14-day filter) returned adjacent leads without methods appraisal. Search filters/index dates are not publication dates. No source added; latest selected first publication remains 26 August 2026. No claim of complete coverage.

Search · 9 Oct 2026

Targeted AG-R05 methods audit following the open step identified on 8 October. Direct PMC access was limited by reCAPTCHA and publisher access by a redirect; no identical retries. Two targeted queries yielded indexed original passages. Also searched DOI+correction/retraction and TIMP2+2026+October: no attributable new correction/retraction notice or new study ready for inclusion found. Hits concerning other papers were not attributed to this study. An institutional profile hit remains an unappraised lead without confirmed DOI/publication metadata. Not comprehensive monitoring; latest selected first publication remains 26 August 2026. Source delta 0; no fresh axolotl audit without a new trigger.

Search · 8 Oct 2026

Reason: open AG-D06 APOE/cis/LD/colocalisation check. Direct PMC request and observed supplement reference return reCAPTCHA today; no specific supplement file read. Three targeted DOI/PMCID+supplement/code queries yielded no verified article-specific code link. Indexed AppendixA points only to the DOI. Earlier publisher/PDF403 not retried. Main run: DOI+correction/retraction and TIMP2 cognition2026 September study; no matching notice, mostly unrelated cognition results, no extra study admitted. No completeness guarantee. Source delta0. Access gap parked pending concrete new access; next separate source check AG-R05.

Search · 7 Oct 2026

Reason: human PRS candidate queued6Oct. Searched DOI and DOI+correction/retraction; assessed final PMC main text, institutional PDF text and PubMed metadata. First online23Oct2025, issueJanuary2026, retrieved7Oct2026. No matching correction/retraction found; no completeness guarantee. PubMed links medRxiv10.1101/2024.07.23.24310854 and ResearchSquare10.21203/rs.3.rs-5267673/v1 as predecessors, not independent evidence. ScienceDirect403 not retried; institutional download403 not repeated. Pixel inspection unsuccessful; tables read as text, supplement/raw data unexamined. No new effect calculation. Source delta+1(AG-D06); no repeat broad axolotl search. Next: inspect existing APOE/cis/LD/colocalisation checks in supplement/code.

Search · 6 Oct 2026

Reason: open direct-group comparison in AG-R08. Indexed eNeuro/PMC original methods, Fig.1/6 legends and conflicts examined; figure subpage unavailable directly, old PMC CAPTCHA not retried. No figure pixels/raw data assessed. Searched DOI with correction/retraction and TIMP2 cognition synapse2026 September; no matching correction notice, no completeness guarantee. Publisher/PMC date discrepancy unresolved; extra date search did not reliably clarify it. One human PRS candidate (10.1016/j.neurobiolaging.2025.10.003) screened from indexed text only; direct publisher403, no retry; exact first date, methods/dependencies next. Source delta0. No own effect calculation; p-values are published statistics.

Search · 5 Oct 2026

Reason: open audit from 4 Oct. Official AG-S03 peer-review file downloaded normally (45 pages), full extracted text searched for Nacu/2016, SAG, ALM, sorting/selection, EGFP, functional; relevant sections read and PDF pages 4, 5, 17, 18, 27, 28, 29, 31, 35, 36 visually checked. Not a line-by-line audit of the whole file. No matched direct Nacu-ED3 comparison found; negative search finding limited to this scope. Revision-letter abnormal digits 4/6 lack final figure/case linkage. Targeted title/DOI correction/retraction check found no matching notice in publisher/PubMed/PMC results; no completeness guarantee. Source delta 0, no new study selected today, no broad search for latest publications or repeated inaccessible tau full texts. No calculation without case linkage. Original file

Search · 4 Oct 2026

Specific reason: unresolved Nacu/Otsuki disagreement from 3 Oct. Exact title/DOI 10.1038/nature17972, primary preview, Extended Data 3 and original supplement inspected; main text from a ResearchGate copy labelled as author-uploaded, without authenticated version comparison. ED3-specific exposure and graft/animal dependencies remain open. AG-S03 methods and Extended Data 10 reviewed for washout/endpoint and cell selection. Targeted Nacu DOI correction/retraction search found no matching notice; not a complete registry audit, commentaries are not corrections. One older primary study added; no new publication today or repeated broad tau search. No cross-study calculation.

Search · 2–3 Oct2026

2 Oct: Jiang DOI/original abstract, version and correction/retraction searches; final publisher 403, no access bypass. Guo reporting form visually inspected. 3 Oct: Crossref/bioRxiv for Jiang versions without explicit relation; author comparison with Guo. AG-S03 full text Figs. 3–5, discussion/methods targeted for spatial control; DOI+correction/retraction found no matching notice (not comprehensive registry audit). Nacu 2016 identified for next countercheck, not fully appraised today. No repeated numerical analysis, no new study on3 Oct; retrieval and publication dates kept separate.

Search · 1 Oct 2026

AG-D01: targeted fragment/epitope audit, main text accessible; supplement errors, no bypass. AG-D03: autophagy/antibody/LC3 and primary DOI; publisher preview, figures and public XLSX inspected. Descriptive rotarod recalculation, not independent replication. DOI/title correction/retraction search found no matching notice; not a complete registry audit. Related DOI 10.1126/scitranslmed.aea4205 identified only as the next appraisal candidate, not counted as deeply reviewed today.

Search · 30 Sep 2026

One additional paper (AG-D02), deeper measurement audit of AG-D01. Searched microglia/tau/degradation/export and quantitative assays; DOI/title plus correction/retraction found no matching notice, not a full registry audit. Shultz: indexed primary methods and figure legends, direct retrieval blocked; preprint not counted separately. Karabova: Figure 4 and methods assessed, supplement access limited. No verified matched dataset for calculating degradation rates.

Search · 29 Sep 2026

Today: targeted methods review of two existing papers and one additional primary study, three sources in total. DOI/title correction and retraction searches found no matching notice; no comprehensive registry audit. Guldner: direct retrieval blocked, indexed publisher sections read. Hemmer: PMC methods read, supplementary figure inaccessible. Karabova: publisher full text accessible. Publication dates are separated from retrieval date in the report. No systematic September coverage.

Targeted narrative search of publisher pages, PubMed and PMC; not a systematic review or meta-analysis. Fourteen primary studies: thirteen peer-reviewed and one preprint. First-publication dates in the selected set reach June 2026; later material has not been comprehensively checked. Older mechanistic studies were retained. No original raw-data analysis or experiments were performed.

Search queries and follow-up verification

• axolotl regeneration 2026 2025 brain protein ageing regeneration primary study
• brain aging protein 2026 rejuvenation Nature study
• axolotl senescence limb regeneration 2025 2026 study
• site.nature.com brain 2025 aging protein Nature original study
• Shifts in protein aggregate stability 2026
• site.nature.com KLK6 2026 aging brain
• site.science.org brain aging microglia 2026 midlife 3D genome
• Axolotl brain 2022 Science regeneration single cell
• Ageing promotes microglial Published
• FTL1 aging hippocampus 2025 Villeda Nature Aging
• axolotl heart regeneration 2025 2026 original study
• Molecular basis positional memory limb regeneration Otsuki 2025 09036
• FTL1 deubiquitinase neuronal aging USP7 redox
• FTL1 deubiquitinase ferritin aging brain DUB
• "Ftl1" "deubiquitylase"
• "FTL1" "USP7" aging

Title, DOI, date and source access were checked separately. The targeted FTL1/DUB prior-art search did not establish novelty. The June 2026 cardiac study was followed up via PMID 42346394. Press and social sources were not used as outcome evidence. Some PubMed/PMC/Elsevier pages were challenge-protected; source-level access limitations are in the register. Full supplements, all power analyses, conflicts of interest and raw data were not audited. Independent replication was generally not assessed. Clinical intervention studies and trial registers were outside this first mechanistic report; their omission is not an assessment of the clinical field.

Next run: new studies, corrections and independent replications; a focused test of the FTL1/redox/DUB hypothesis. Preserve negative searches and conflicting evidence.

Continue exploring

Approaches to aging

Evidence and functional outcomes

→

Evidence chain

How studies generate new questions

Research colloquium

16 foundation assignments + 4 new deep dives

a.AGINGRESEARCH IN PROGRESS

Project overviewGoals & methodsEvidence chain

AI-assisted literature research and model building. No laboratory or clinical studies conducted by this project. No personal treatment plans.

Image credits · Illustrations, not project data: Axolotl — LoKiLeCh (CC BY-SA 3.0) · Neuron — explorebiology (Explore Biology) (CC BY 4.0) · DNA — Biochemlife (CC BY-SA 4.0) · Bovine endothelial cells — Erin Rod (CC BY 4.0)